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Rabbit Anti-Bcl-2 antibody (bs-0032R)
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说明书: 50ul  100ul  200ul
20ul/580.00元
50ul/980.00元
100ul/1680.00元
200ul/2480.00元
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产品编号 bs-0032R
英文名称 Bcl-2
中文名称 Bcl-2抗体
别    名 Apoptosis regulator Bcl 2; Apoptosis regulator Bcl2; AW986256; B cell CLL/lymphoma 2; B cell leukemia/lymphoma 2; B cell lymphoma 2; Bcl 2; Bcl-2; Bcl2; BCL2 protein; C430015F12Rik; D630044D05Rik; D830018M01Rik; Leukemia/lymphoma, B-cell, 2; Oncogene B-cell leukemia 2; BCL2_HUMAN.  
Specific References  (53)     |     bs-0032R has been referenced in 53 publications.
[IF=0] Zhou et al. Azithromycin synergistically enhances anti-proliferative activity of vincristine in cervical and gastric cancer cells. (2012) Cancers.(Basel). 4:1318-32  WB ;  Human.  
[IF=6.434] Yu D et al. Gestational exposure to acrylamide inhibits mouse placental development in vivo.(2018) J Hazard Mater.367:160-170.   WB ;  Mouse.  
[IF=6.375] Zhou,et al.CXCR4 antagonist AMD3100 enhances the response of MDA-MB-231 triple-negative breast cancer cells to ionizing radiation.(2018) Cancer Letters. 418:196-203.  IHC-P + WB ;  Mouse.  
[IF=6.217] Wang L et al. Zoledronic acid inhibits the growth of cancer stem cell derived from cervical cancer cell by attenuating their stemness phenotype and inducing apoptosis and cell cycle arrest through the Erk1/2 and Akt pathways. J Exp Clin Cancer Res. 2019 Feb 21;38(1):93.  WB ;  Mouse.  
[IF=4.686] Sun,et al.Exogenous H2S switches cardiac energy substrate metabolism by regulating SIRT3 expression in db/db mice.(2018) Journal of Molecular Medicine. 96:281-299.  WB ;  Mouse.  
[IF=4.59] Qiao et al. Polydatin Attenuates H2O2-Induced Oxidative Stress via PKC Pathway. (2016) Oxid.Med.Cell.Longe. 2016:5139458  WB ;  Human.  
[IF=4.59] Qiao et al. Polydatin Attenuates H2O2-Induced Oxidative Stress via PKC Pathway. (2016) Oxid.Med.Cell.Longe. 2016:5139458  WB ;  Human.  
[IF=3.909] Zhu B et al. The hepatoprotective effect of polysaccharides from Pleurotus ostreatus on carbon tetrachloride-induced acute liver injury rats.Int J Biol Macromol. 2019 Jun 15;131:1-9.   IHF ;  Rat.  
[IF=3.775] Chen Z et al. Final-2 targeted glycolysis mediated apoptosis and autophagy in human lung adenocarcinoma cells but failed to inhibit xenograft in nude mice. Food Chem Toxicol. 2019 Aug;130:1-11.   WB ;  Human.  
[IF=3.775] Chen Z et al. Final-2 targeted glycolysis mediated apoptosis and autophagy in human lung adenocarcinoma cells but failed to inhibit xenograft in nude mice. Food Chem Toxicol. 2019 Aug;130:1-11.   WB ;  Human.  
[IF=3.616] Geng X et al. Role of ZIP8 in regulation of cisplatin sensitivity through Bcl-2. (2018) Toxicol. Appl. Pharmacol. Oct 17;362.   IF ;  Mouse.  
[IF=3.457] Chai FN et al. Coptisine from Rhizoma coptidis exerts an anti-cancer effect on hepatocellular carcinoma by up-regulating miR-122.Biomed Pharmacother. 2018 Jul;103:1002-1011.  WB ;  Mouse.  
[IF=3.44] Chen B et al. Inhibition of Connexin43 Hemichannels with Gap19 Protect-s Cerebral Ischemia/Reperfusion Injury via the JAK2/STAT3 Pathway in Mice.(2018) Brain Res Bull.  WB ;  Mouse.  
[IF=3.412] Chen S et al. Anthocyanins from Lycium ruthenicum Murr. ameliorated D-galactose-induced memory impairment, oxidative stress, and neuroinflammation in adult rats.J Agric Food Chem. 2019 Mar 20;67(11):3140-3149.  WB&IHC-P ;  Rat.  
[IF=3.33] Chen, Jie, et al. "Targeting SPARC by lentivirus-mediated RNA interference inhibits cervical cancer cell growth and metastasis." BMC cancer 12.1 (2012): 464.  WB ;  Human.  
[IF=3.33] Tian, Xiao Qiang, et al. "The protective effect of Hyperbaric oxygen and Ginkgo Biloba extract on Aβ25-35-induced oxidative stress and neuronal apoptosis in rats." Behavioural Brain Research (2012).  WB ;  Rat.  
[IF=3.288] Chai D et al. β2-microglobulin has a different regulatory molecular mechanism between ER+ and ER- breast cancer with HER2.BMC Cancer. 2019 Mar 12;19(1):223.  IHC-P ;  Human.  
[IF=3.208] Cui H et al. Gamma linolenic acid regulates PHD2 mediated hypoxia and mitochondrial apoptosis in DENinduced hepatocellular carcinoma. Drug Des Devel Ther. 2018 Dec 13;12:4241-4252.   WB ;  Rat.  
[IF=3.138] Chen J et al. Effect of artemisinin on proliferation and apoptosis-related protein expression in vivo and in vitro.Saudi J Biol Sci. 2018 Nov;25(7):1488-1493.  WB&IHC ;  Rat.  
[IF=3.13] Zhang, Wen-feng, et al. "Angelica polysaccharides inhibit the growth and promote the apoptosis of U251 glioma cells in vitro and in vivo." Phytomedicine (2017).  WB ;  Human.  
[IF=3.105] Yang F et al. Copper induces oxidative stress and apoptosis through mitochondria-mediated pathway in chicken hepatocytes.(2019)Toxicol In Vitro. Feb;54:310-316.   WB ;  Chicken.  
[IF=3.061] Zhu H et al. Catalpol improves axonal outgrowth and reinnervation of injured sciatic nerve by activatingAkt/mTOR pathway and regulating BDNF and PTEN expression. Am J Transl Res. 2019 Mar 15;11(3):1311-1326.  WB ;  Mouse.  
[IF=2.85] Li et al. A plasma mir-125a-5p as a novel biomarker for Kawasaki disease and induces apoptosis in HUVECs. (2017) PLoS.On. 12:e0175407  WB ;  Human.  
[IF=2.784] Zhang et al. Sphingomyelin synthase 2 promotes H2O2-induced endothelial dysfunction by activating the Wnt/β-catenin signaling pathway. (2018) Int.J.Mol.Med. 42:3344-3354  WB ;  
[IF=2.784] Zhang P et al. Sphingomyelin synthase 2 promotes H2O2-induced endothelial dysfunction by activating the Wnt/β-catenin signaling pathway. (2018) Int J Mol Med. 2018 Sep 19.   WB ;  
[IF=2.76] Liu, Qiang, et al. "Valproic acid attenuates inflammation of optic nerve and apoptosis of retinal ganglion cells in a rat model of optic neuritis." Biomedicine & Pharmacotherapy(2017).  WB ;  Rat.  
[IF=2.656] Liu et al. Effects of insulin-like growth factor binding protein 3 on apoptosis of cutaneous squamous cell carcinoma cells. (2018) Onco.Targets.Ther. 11:6569-6577  WB ;  
[IF=2.589] Yin G et al. Effect of 5-aminolevulinic acid photodynamic therapy on the expression of apoptosis inhibitors Bcl-2 and Survivin in keratinocytes of condyloma acuminatum . Photodiagnosis Photodyn Ther. 2019 Jul 23. pii: S1572-1000(19)30016-X.   IHC ;  Human.  
[IF=2.561] Peng X et al. Overexpression of modified human TRβ1 suppresses the growth of hepatocarcinoma SK-hep1 cells in vitro and in xenograft models.Mol Cell Biochem. 2018 Dec;449(1-2):207-218.   IHC-P&WB ;  Mouse&Human.  
[IF=2.559] Jia C et al. Enhanced antitumor effect of combination of annexin A1 knockdown and bortezomib treatment in multiple myeloma in vitro and in vivo(2018) Biochem Biophys Res Commun.505(3):720-725  WB ;  Mouse.  
[IF=2.38] She, Yue, et al. "Aluminum Trichloride Disorders Bile Acid Secretion and Induces Hepatocyte Apoptosis in Rats." Cell Biochemistry and Biophysics (2015): 1-9.  IHC-P ;  Rat.  
[IF=2.341] Ling,et al.Long noncoding RNA ENSRNOG00000037522 is involved in the podocyte epithelial‑mesenchymal transition in diabetic rats.(2018) International Journal of Molecular Medicine. 41:2704-2714.  IHC-P ;  Rat.  
[IF=2.27] He, X., et al. "Rapamycin inhibits acrolein‐induced apoptosis by alleviating ROS‐driven mitochondrial dysfunction in male germ cells." Cell Proliferation (2014).  WB ;  Mouse.  
[IF=1.949] Li Q et al. The effects of photobiomodulation therapy on mouse pre-osteoblast cell line MC3T3-E1 proliferation and apoptosis via miR-503/Wnt3a pathway.(2018) Lasers Med Sci. Sep 15  WB ;  mouse.  
[IF=1.922] Yong et al. Effects of compound 21, a non‑peptide angiotensin II type 2 receptor agonist, on general anesthesia‑induced cerebral injury in neonatal rats. (2018) Mol.Med.Rep. 18:5337-5344  WB ;  Rat.  
[IF=1.894] Chi et al. Tail Vein Infusion of Adipose-Derived Mesenchymal Stem Cell Alleviated Inflammatory Response and Improved Blood Brain Barrier Condition by Suppressing Endoplasmic Reticulum Stress in a Middle Cerebral Artery Occlusion Rat Model. (2018) Med.Sci.Monit. 24:3946-3957  WB ;  Rat.  
[IF=1.652] Wang Y et al. Protective effects of astaxanthin on subarachnoid hemorrhage-induced early brain injury: Reduction of cerebral vasospasm and improvement of neuron survival and mitochondrial function.(2019)Acta Histochem. Jan;121(1):56-63.   WB ;  Rat.  
[IF=1.55] Yang, Jinjiang, Ying Lu, and Ai Guo. "Platelet-rich plasma protects rat chondrocytes from interleukin-1β-induced apoptosis." Molecular Medicine Reports 14.5 (2016): 4075-4082.  WB ;  Rat.  
[IF=1.39] Wang et al. Human cytomegalovirus inhibits apoptosis by regulating the activating transcription factor 5 signaling pathway in human malignant glioma cells. (2014) Oncol.Let. 8:1051-1057  WB ;  Human.  
[IF=1.39] Wang et al. Human cytomegalovirus inhibits apoptosis by regulating the activating transcription factor 5 signaling pathway in human malignant glioma cells. (2014) Oncol.Let. 8:1051-1057  WB ;  Human.  
[IF=1.173] Xu et al. Danshen attenuates cartilage injuries in osteoarthritis in vivo and in vitro by activating JAK2/STAT3 and AKT pathways. (2018) Exp.Anim. 67:127-137  WB ;  Rabbits.  
[IF=.181] Zhu HF et al. Catalpol enhances neurogenesis in the SVZ in a rat model of strokes. Int J Clin Exp Med 2019;12(7):8068-8077.  WB ;  Rat.  
[IF=0] Li et al. Morusin suppresses breast cancer cell growth in vitro and in vivo through C/EBPβ and PPARγ mediated lipoapoptosis. (2015) J.Exp.Clin.Cancer.Res. 34:137  WB ;  Human.  
[IF=0] Zhang et al. Protective Effects of Lycium barbarum Polysaccharides on Testis Spermatogenic Injury Induced by Bisphenol A in Mice. (2014) Evid.Based.Complement.Alternat.Me. 2013:690808  IHC-P ;  Mouse.  
[IF=0] Han et al. Protective effects of tao-Hong-si-wu decoction on memory impairment and hippocampal damage in animal model of vascular dementia. (2015) Evid.Based.Complement.Alternat.Me. 2015:195835  IHC-P ;  Rat.  
[IF=0] Ma et al. A potential adjuvant chemotherapeutics, 18β-glycyrrhetinic acid, inhibits renal tubular epithelial cells apoptosis via enhancing BMP-7 epigenetically through targeting HDAC2. (2016) Sci.Re. 6:25396  WB ;  Human,Mouse.  
[IF=0] Hermawati E et al. Spatial Memory Disturbance Following Transient Brain Ischemia is Associated with Vascular Remodeling in Hippocampus. Kobe J Med Sci. 2018 Oct 15;64(3):E93-E106.  IHC-P ;  Rat.  
[IF=0] Gao Y et al. Hepatoprotective effects of limb ischemic post-conditioning in hepatic ischemic rat model and liver cancer patients via PI3K/ERK pathways.(2018)Int J Biol Sci. Nov 3;14(14):2037-2050.   IHC ;  Rat.  
[IF=0] Li X et al. High level expression of ISG12 (1) promotes cell apoptosis via mitochondrial-dependent pathway and so as to hinder Newcastle disease virus replication.(2019)Vet Microbiol. Jan;228:147-156.   WB ;  Chicken.  
[IF=0] Zhang Y et al. Ginsenoside Rg3 prevents cognitive impairment by improving mitochondrial dysfunction in the rat model of Alzheimer's disease. J Agric Food Chem. 2019 Aug 27.   WB&IHC-P ;  Rat.  
[IF=8.063] Luo J et al. ATF4 destabilizes RET through nonclassical GRP78 inhibition to enhance chemosensitivity to bortezomib in human osteosarcoma. Theranostics. 2019 Aug 14;9(21):6334-6353.   IHC-P ;  Human.  
[IF=1.07] Wei X et al. c [RGDyk]-coated liposomes loaded with adriamycin and miR-21 mimics inhibit the growth of hepatoma cell line SMCC-7721 via up-regulating Bax and p53. Transl Cancer Res 2019;8(4):1311-1318.  WB ;  Human.  
[IF=0] Chen S et al. Anthocyanins from Lycium ruthenicum Murr. Ameliorated d-Galactose-Induced Memory Impairment, Oxidative Stress, and Neuroinflammation in Adult Rats. J Agric Food Chem. 2019 Mar 20;67(11):3140-3149.   WB ;  Rat.  
研究领域 细胞生物  信号转导  细胞凋亡  细胞类型标志物  肿瘤细胞生物标志物  新陈代谢  线粒体  
抗体来源 Rabbit
克隆类型 Polyclonal
交叉反应 Human, Chicken, Dog, Pig, Cow, Horse, Rabbit, Guinea Pig, 
产品应用 WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100 IF=1:100-500 (石蜡切片需做抗原修复)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量 26kDa
细胞定位 细胞核 细胞浆 细胞膜 线粒体
性    状 Liquid
浓    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from human Bcl-2:101-160/236 
亚    型 IgG
纯化方法 affinity purified by Protein A
储 存 液 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件 Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
PubMed PubMed
产品介绍 The Bcl-2 gene was isolated at the chromosomal breakpoint of t(14;18)-bearing follicular B cell lymphomas(1,2).Bcl-2 blocks cell death following a variety of stimuli and confers a death-sparing effect to certain hematopoietic cell lines following growth factor withdrawal (3,5).Bcl-2 appears to function in several subcellular locations yet lacks any known motifs that would confer insight into its mechanism of action (6,7).A more recently identified protein,designated Bax p21(i.e., Bcl-associated X protein ),has extensive amino acid homology with Bcl-2 and both homodimerizes and forms heterodimers with Bcl-2(8). Overexpression of Bax accelerates apoptotic death induced by cytokine deprivation in an IL-3 dependent cell line and Bax also counters the death repressor activty of Bcl-2(8).

Function:
Suppresses apoptosis in a variety of cell systems including factor-dependent lymphohematopoietic and neural cells. Regulates cell death by controlling the mitochondrial membrane permeability. Appears to function in a feedback loop system with caspases. Inhibits caspase activity either by preventing the release of cytochrome c from the mitochondria and/or by binding to the apoptosis-activating factor (APAF-1).

Subunit:
Forms homodimers, and heterodimers with BAX, BAD, BAK and Bcl-X(L). Heterodimerization with BAX requires intact BH1 and BH2 motifs, and is necessary for anti-apoptotic activity. Interacts with EI24 (By similarity). Also interacts with APAF1, BBC3, BCL2L1, BNIPL, MRPL41 and TP53BP2. Binding to FKBP8 seems to target BCL2 to the mitochondria and probably interferes with the binding of BCL2 to its targets. Interacts with BAG1 in an ATP-dependent manner. Interacts with RAF1 (the 'Ser-338' and 'Ser-339' phosphorylated form). Interacts (via the BH4 domain) with EGLN3; the interaction prevents the formation of the BAX-BCL2 complex and inhibits the anti-apoptotic activity of BCL2. Interacts with G0S2; this interaction also prevents the formation of the anti-apoptotic BAX-BCL2 complex.

Subcellular Location:
Mitochondrion outer membrane; Single-pass membrane protein. Nucleus membrane; Single-pass membrane protein. Endoplasmic reticulum membrane; Single-pass membrane protein.

Tissue Specificity:
Expressed in a variety of tissues.

Post-translational modifications:
Phosphorylation/dephosphorylation on Ser-70 regulates anti-apoptotic activity. Growth factor-stimulated phosphorylation on Ser-70 by PKC is required for the anti-apoptosis activity and occurs during the G2/M phase of the cell cycle. In the absence of growth factors, BCL2 appears to be phosphorylated by other protein kinases such as ERKs and stress-activated kinases. Phosphorylated by MAPK8/JNK1 at Thr-69, Ser-70 and Ser-87, wich stimulates starvation-induced autophagy. Dephosphorylated by protein phosphatase 2A (PP2A).
Proteolytically cleaved by caspases during apoptosis. The cleaved protein, lacking the BH4 motif, has pro-apoptotic activity, causes the release of cytochrome c into the cytosol promoting further caspase activity.
Monoubiquitinated by PARK2, leading to increase its stability.

DISEASE:
Note=A chromosomal aberration involving BCL2 has been found in chronic lymphatic leukemia. Translocation t(14;18)(q32;q21) with immunoglobulin gene regions. BCL2 mutations found in non-Hodgkin lymphomas carrying the chromosomal translocation could be attributed to the Ig somatic hypermutation mechanism resulting in nucleotide transitions.

Similarity:
Belongs to the Bcl-2 family.

SWISS:
P49950

Gene ID:
596

Database links:

Entrez Gene: 281020 Cow

Entrez Gene: 596 Human

Entrez Gene: 12043 Mouse

Entrez Gene: 24224 Rat

Omim: 151430 Human

SwissProt: O02718 Cow

SwissProt: P10415 Human

SwissProt: P10417 Mouse

SwissProt: P49950 Rat

Unigene: 150749 Human

Unigene: 257460 Mouse

Unigene: 9996 Rat



Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

Bcl-2基因是指B-cell lymphoma gene。人体滤泡B细胞淋巴瘤中过量表达的原癌基因。由于染色体t(14;18)易位,将Bcl-2基因置于免疫球蛋白重链的转录调控下,使其表达失控。在细胞系中其过量表达能延长细胞存活期而不诱导细胞增殖。它是哺乳动物中细胞调亡的抑制基因。参与细胞凋亡的调控。肿瘤中的Bcl-2基因可提高侵润性瘤细胞的生存能力。主要用于滤胞型淋巴瘤、毛细管性白血病及细胞凋亡等方面的研究。

目前研究认为:Bcl-2也是细胞凋亡的一种抑制因子、参与细胞凋亡调控,可以用于各种恶性肿瘤的细胞凋亡的研究。
产品图片
Protein: Spleen(Mouse)lysate 30ug;
Primary: Anti-Bcl-2(bs-0032R) at 1:300;
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/10000 dilution
Predicted band size : 26kD
Observed band size : 26kD
Protein: Brain(Mouse)lysate 30ug;
Primary: Anti-Bcl-2(bs-0032R) at 1:200;
Secondary: HRP conjugated Goat Anti-Rabbit IgG(bs-0295G-HRP) at 1: 5000;
Predicted band size : 26kD
Observed band size : 26kD
Sample:
Bcl-2 overexpression E.coli lysate (whole cell) Lysate at 40 ug
Recombinant protein Lysate at 40 ug
Primary: Anti-Bcl-2 (bs-0032R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 26 kD
Observed band size: 25 kD
Sample:Spleen (Mouse) Lysate at 40 ug
Primary: Anti-Bcl-2 (bs-0032R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 26 kD
Observed band size: 26 kD
Sample:
MCF-7 (Human) Cell Lysate at 30 ug
Hela (Human) Cell Lysate at 30 ug
HL60 (Human) Cell Lysate at 30 ug
A431 (Human) Cell Lysate at 30 ug
Primary: Anti-Bcl-2 (bs-0032R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 26 kD
Observed band size: 23 kD
Sample:
Jurkat(Human) Cell Lysate at 30 ug
Primary: Anti-Bcl-2 (bs-0032R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 26 kD
Observed band size: 26 kD
Sample:
Spleen (Mouse) Lysate at 40 ug
RAW264.7 Cell (Mouse) Lysate at 40 ug
Primary: Anti-Bcl-2 (bs-0032R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 26 kD
Observed band size: 26 kD
Paraformaldehyde-fixed, paraffin embedded (human pancreatic cancer); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Bcl-2) Polyclonal Antibody, Unconjugated (bs-0032R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human pancreatic cancer); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Bcl-2) Polyclonal Antibody, Unconjugated (bs-0032R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Rat spinal cord); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Bcl-2) Polyclonal Antibody, Unconjugated (bs-0032R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Bcl-2) Polyclonal Antibody, Unconjugated (bs-0032R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Bcl-2) Polyclonal Antibody, Unconjugated (bs-0032R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat ovary tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Bcl-2) Polyclonal Antibody, Unconjugated (bs-0032R) at 1:400 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
Tissue/cell: MCF-7 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (Bcl-2) Polyclonal Antibody, Unconjugated (bs-0032R) 1:50, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody (bs-0295G-Cy3) at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
Paraformaldehyde-fixed, paraffin embedded (Rat thyroid gland); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (B cell lymphoma 2; Bcl-2) Polyclonal Antibody, Unconjugated (bs-0032R) at 1:200 overnight at 4°C, followed by a conjugated secondary antibody (bs-0295G-cy3) for 90 minutes and DAPI for nuclei staining.
Tissue/cell: Hela cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (Bcl-2) polyclonal Antibody, Unconjugated (bs-0032R) 1:100, 90 minutes at 37°C; followed by a FITC conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
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