永利线路检测

bs-0061R [Primary Antibody]
Rabbit  Anti-beta-Actin (Loading Control) Polyclonal Antibody
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Host: Rabbit

Target Protein: beta-Actin (Loading Control)

IR: Immunogen Range:1-200/375

Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 60

Swiss Prot: P60709

Source: Synthetic MAP peptide derived from human beta-Actin:1-200/375 

Purification: affinity purified by Protein A

Storage: 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Background: Loading Control
This gene encodes one of six different actin proteins. Actins are highly conserved proteins that are involved in cell motility, structure, and integrity. This actin is a major constituent of the contractile apparatus and one of the two nonmuscle cytoskeletal actins. [provided by RefSeq, Jul 2008].

Size: 200ul

Concentration: 1mg/ml

Applications: WB(1:5000-20000)
ELISA(1:5000-20000)
IHC-P(1:500-1000)
Flow-Cyt(1μg/Test)
ICC(1:100)

Cross Reactive Species: Human
Mouse
Rat
Chicken
Dog
Rabbit
Sheep
Bee
Fish
Guinea Pig
Hamster
Cat
mt,op.

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES
Sample:
Lung (Mouse) Lysate at 40 ug
Uterus (Mouse) Lysate at 40 ug
Small intestine (Mouse) Lysate at 40 ug
Primary: Anti- beta-Actin (bs-0061R) at 1/2000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 42 kD
Observed band size: 42 kD
Sample:
A549 Cell (Human) Lysate at 30 ug
Primary: Lane1: Anti-beta-Actin (bs-0061R) at 1/500 dilution
Lane2: Anti-beta-Actin (bs-0061R) at 1/1000 dilution
Lane3: Anti-beta-Actin (bs-0061R) at 1/2000 dilution
Lane4: Anti-beta-Actin (bs-0061R) at 1/4000 dilution
Lane5: Anti-beta-Actin (bs-0061R) at 1/5000 dilution
Lane6: Anti-beta-Actin (bs-0061R) at 1/8000 dilution
Lane7: Anti-beta-Actin (bs-0061R) at 1/10000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 42 kD
Observed band size: 42 kD
Sample:
A549 Cell (Human) Lysate at 30 ug
Primary: Lane1: Anti-beta-Actin (bs-0061R) at 1/500 dilution
Lane2: Anti-beta-Actin (bs-0061R) at 1/1000 dilution
Lane3: Anti-beta-Actin (bs-0061R) at 1/2000 dilution
Lane4: Anti-beta-Actin (bs-0061R) at 1/4000 dilution
Lane5: Anti-beta-Actin (bs-0061R) at 1/5000 dilution
Lane6: Anti-beta-Actin (bs-0061R) at 1/8000 dilution
Lane7: Anti-beta-Actin (bs-0061R) at 1/10000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 42 kD
Observed band size: 42 kD
Sample:
Lane1: 293T Cell Lysate at 25 ug
Lane2: A549 Cell Lysate at 25 ug
Lane3: A431 Cell Lysate at 25 ug
Primary: Anti- beta-Actin (bs-0061R) at 1/1000 and 1/5000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 42kD
Observed band size: 42 kD
Sample: Lung lysate at 30ug;
Primary: Anti-beta-actin (bs-0061R) at 1:1000 dilution
Secondary: HRP conjugated Goat-Anti-Rabbit IgG(bse-0295G) at 1:3000 dilution
Predicted band size : 42kD
Observed band size : 42kD
Sample:
Lane 1: SH-SY5Y (Human) Lysate at 40 ug
Lane 2: SH-SY5Y (Human) Lysate at 40 ug
Lane 3: SH-SY5Y (Human) Lysate at 40 ug
Lane 4: SH-SY5Y (Human) Lysate at 40 ug
Primary:
Lane 1: Anti-beta-Actin (bs-0061R) at 1/2000 dilution
Lane 2: Anti-beta-Actin (bs-0061R) at 1/5000 dilution
Lane 3: Anti-beta-Actin (bs-0061R) at 1/10000 dilution
Lane 4: Anti-beta-Actin (bs-0061R) at 1/20000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 42 kD
Observed band size: 42 kD
Sample:
Lane 1: Lymph node (Rat) Lysate at 40 ug
Lane 2: Lymph node (Rat) Lysate at 40 ug
Lane 3: Lymph node (Rat) Lysate at 40 ug
Lane 4: Lymph node (Rat) Lysate at 40 ug
Lane 5: Lymph node (Rat) Lysate at 40 ug
Primary:
Lane 1: Anti-beta-Actin (bs-0061R) at 1/1000 dilution
Lane 2: Anti-beta-Actin (bs-0061R) at 1/2000 dilution
Lane 3: Anti-beta-Actin (bs-0061R) at 1/5000 dilution
Lane 4: Anti-beta-Actin (bs-0061R) at 1/10000 dilution
Lane 5: Anti-beta-Actin (bs-0061R) at 1/20000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 42 kD
Observed band size: 42 kD
Sample:
Lane 1: Thymus (Mouse) Lysate at 40 ug
Lane 2: Thymus (Mouse) Lysate at 40 ug
Lane 3: Thymus (Mouse) Lysate at 40 ug
Lane 4: Thymus (Mouse) Lysate at 40 ug
Lane 5: Thymus (Mouse) Lysate at 40 ug
Primary:
Lane 1: Anti-beta-Actin (bs-0061R) at 1/1000 dilution
Lane 2: Anti-beta-Actin (bs-0061R) at 1/2000 dilution
Lane 3: Anti-beta-Actin (bs-0061R) at 1/5000 dilution
Lane 4: Anti-beta-Actin (bs-0061R) at 1/10000 dilution
Lane 5: Anti-beta-Actin (bs-0061R) at 1/20000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 42 kD
Observed band size: 42 kD
Sample:
HL60 (Human) Cell Lysate at 40 ug
HUVEC (Human) Cell Lysate at 40 ug
Spinal cord (Rat) Lysate at 40 ug
Rectum (Rat) Lysate at 40 ug
Placenta (Rat) Lysate at 40 ug
Lymph node (Rat) Lysate at 40 ug
Lung (Rat) Lysate at 40 ug
Spleen (Rat) Lysate at 40 ug
JAR (Human) Cell Lysate at 40 ug
293T (Human) Cell Lysate at 40 ug
Jurkat (Human) Cell Lysate at 40 ug
TM4 (Human) Cell Lysate at 40 ug
Primary: Anti-beta-Actin (bs-0061R) at 1/2000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 42 kD
Observed band size: 42 kD
Sample:
A549 (Human) Cell Lysate at 40 ug
Raw264.7 (Mouse) Cell Lysate at 40 ug
SH-SY5Y (Human) Cell Lysate at 40 ug
MKN45 (Human) Cell Lysate at 40 ug
CHO (Human) Cell Lysate at 40 ug
Panc-1 (Human) Cell Lysate at 40 ug
4T1 (Human) Cell Lysate at 40 ug
ASPC-1 (Human) Cell Lysate at 40 ug
H9C2 (Rat) Cell Lysate at 40 ug
Brl-3a (Rat) Cell Lysate at 40 ug
TT (Human) Cell Lysate at 40 ug
TEV-1 (Human) Cell Lysate at 40 ug
EC9706 (Human) Cell Lysate at 40 ug
Primary: Anti-beta-Actin (bs-0061R) at 1/2000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 42 kD
Observed band size: 42 kD
Sample:
Embryo Cerebrum (Mouse) Lysate at 40 ug
Du145 (Human) Lysate at 40 ug
SW480 (Human) Cell Lysate at 40 ug
U87MG (Human) Lysate at 40 ug
U251 (Human) Lysate at 40 ug
A673 (Human) Lysate at 40 ug
Lovo (Human) Lysate at 40 ug
293FT (Human) Lysate at 40 ug
JEG-3 (Human) Lysate at 40 ug
RSC96 (Rat) Cell Lysate at 40 ug
MCF-7 (Human) Cell Lysate at 40 ug
HepG2 (Human) Lysate at 40 ug
A431 (Human) Lysate at 40 ug
Primary: Anti-beta-Actin (bs-0061R) at 1/2000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 42 kD
Observed band size: 42 kD
Sample:
Thymus (Mouse) Lysate at 40 ug
Urinary bladder (Mouse) Lysate at 40 ug
Uterus (Mouse) Cell Lysate at 40 ug
Aorta (Mouse) Lysate at 40 ug
olfactory bulb (Mouse) Lysate at 40 ug
Cerebellum (Mouse) Lysate at 40 ug
Adrenal gland (Mouse) Lysate at 40 ug
Ovary (Mouse) Lysate at 40 ug
Ear (Mouse) Lysate at 40 ug
U2os (Human) Cell Lysate at 40 ug
ASPC-1 (Human) Cell Lysate at 40 ug
Vas deferens (Mouse) Lysate at 40 ug
trachea (Mouse) Lysate at 40 ug
Primary: Anti-beta-Actin (bs-0061R) at 1/2000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 42 kD
Observed band size: 42 kD
Sample:
Lung (Mouse) Lysate at 40 ug
Cerebrum (Mouse) Lysate at 40 ug
Primary: Anti-beta-Actin (Loading Control) (bs-0061R) at 1/2000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 42 kD
Observed band size: 42 kD
Tissue/cell: human cervical carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-Beta-actin Polyclonal Antibody, Unconjugated(bs-0061R) 1:1500, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Tissue/cell: Hela cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (beta-Actin) polyclonal Antibody, Unconjugated (bs-0061R) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG-FITC antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
MCF7 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (beta-Actin) polyclonal Antibody, Unconjugated (bs-0061R) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
MCF7 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (beta-Actin) polyclonal Antibody, Unconjugated (bs-0061R) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
Blank control: NIH/3T3.
Primary Antibody (green line): Rabbit Anti-beta-Actin (Loading Control) antibody (bs-0061R)
Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody : Goat anti-rabbit IgG-AF488
Dilution: 1μg /test.
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at -20℃. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
Blank control:Mouse spleen.
Primary Antibody (green line): Rabbit Anti-beta-Actin (Loading Control) antibody (bs-0061R)
Dilution: 2μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody : Goat anti-rabbit IgG-AF647
Dilution: 1μg /test.
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at-20℃. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
Blank control: A431.
Primary Antibody (green line): Rabbit Anti-ACTB antibody (bs-0061R-AF488)
Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with PBST for 20 min at room temperature. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. Acquisition of 20,000 events was performed.
Blank control: A431.
Primary Antibody (green line): Rabbit Anti-ACTB antibody (bs-0061R-AF488)
Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with PBST for 20 min at room temperature. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. Acquisition of 20,000 events was performed.
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